Mouse Anti-HAVCR1 Recombinant Antibody (TAB-664CT)
CAT#: TAB-664CT
This product is a recombinant mouse antibody that recognizes TIM-1. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7173/if_selected.jpg
Normal Tissue
Figure 2 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75697/159393_A_7_3.jpg
Normal Tissue
Figure 3 Rectum
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75697/159393_A_4_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75697/159393_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75697/159393_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75697/159393_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (A549, CACO-2, RPTEC TERT1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113249-HAVCR1
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SDS-PAGE
Figure 1 Mouse Anti-HAVCR1 Recombinant Antibody (TAB-664CT) in SDS-PAGE
SDS-PAGE analysis of TAB-664CT in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 180-245 kDa.
SEC-HPLC
Figure 2 Mouse Anti-HAVCR1 Recombinant Antibody (TAB-664CT) in SEC-HPLC
The purity of TAB-664CT was 99.80% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
ELISA
Figure 3 Mouse Anti-HAVCR1 Recombinant Antibody (TAB-664CT) in ELISA
ELISA analysis of TAB-664CT was performed by coating with human TIM-1 / HAVCR1 protein, His tag. Then blocked with BSA and incubated with TAB-664CT. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 4 Mouse Anti-HAVCR1 Recombinant Antibody (TAB-664CT) in WB
Western blot analysis of TAB-664CT was performed by loading human TIM-1 / HAVCR1 protein, His tag (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-664CT and HRP goat anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
DB
Figure 5 Mouse Anti-HAVCR1 Recombinant Antibody (TAB-664CT) in DB
Dot Blot analysis of TAB-664CT was performed by coating with human TIM-1 / HAVCR1 protein, His tag.
TAB-664CT incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)
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Specifications
- Immunogen
- Human TIMl-Ig
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human TIM-1
- Species Reactivity
- Human
- Applications
- FC, IB, Block, Inhib, Funcs, ELISA, FACS, IP, IF
- Related Disease
- Inflammatory and autoimmune conditions
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- HAVCR1; hepatitis A virus cellular receptor 1; TIM; KIM1; TIM1; HAVCR; KIM-1; TIM-1; TIMD1; TIMD-1; HAVCR-1; kidney injury molecule 1; T-cell membrane protein 1; T-cell immunoglobulin mucin receptor 1; T-cell immunoglobulin mucin family member 1; T cell immunoglobin domain and mucin domain protein 1; ARD5
- Gene ID
- 26762
- UniProt ID
- Q96D42
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-664CT, RRID: AB_3111982)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| HPAB-0192CQ-F(E) | Human Anti-HAVCR1 Recombinant Antibody; Fab Fragment (HPAB-0192CQ-F(E)) | ELISA, IHC, FC | Human Fab |
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